This protocol was adapted from “Stage 1: Synthesis of first strand cDNA catalyzed by reverse transcriptase” and “Stage 2: Second strand synthesis,” in Molecular Cloning: A Laboratory ...

After hours: February 7 at 4:20:00 PM EST Loading Chart for CDNA ...

The reverse transcription reaction was carried out under the following conditions: primer annealing at 25°C for 2 min, cDNA synthesis at 55°C for 30 min, and heat inactivation at 95°C for 1 min. The ...

Objective: Electrospun nanofibers exhibit potential as scaffolds for articular cartilage tissue regeneration. This study aimed to fabricate electrospun polycaprolactone (PCL)/silk fibroin (SF) ...

A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.

Using an ABI7700 analytical thermal cycler (Perkin-Elmer Applied Biosystem, Foster City, CA), cDNA was measured and TaqMan rodent GAPDH control reagent (Perkin-Elmer Applied Biosystem) was used to ...

In this manuscript the authors established a novel three-dimensional culture system for stratified epithelia that allows epithelial cells to undergo epithelial-to-mesenchymal transition (EMT) and ...