Another study focused on the directed evolution of mCherry, a popular red fluorescent protein. By selecting variants based on fluorescence lifetime, researchers developed a brighter variant ...

For quantification of total mitochondrial EGFP fluorescence per cell according to segmented Tom20-mCherry fluorescence signal. Raw fluorescence images used for quantification of total mitochondrial ...

For AAV-TRE-Kir2.1-T2A-GFP, we amplified the sequences of Kir2.1-T2A-GFP from the pAAV hSyn ... Diagram illustrating the injection of AAV-hMAG-mCherry and AAV-hMAG-DTA into the mouse cerebellum at ...

A, Diagram illustrating the injection of AAV-hMAG-mCherry and AAV-hMAG-DTA into the mouse cerebellum at early postnatal days (P6-7). B, Fluorescence microscopy image showing mCherry expression (red) ...

Cell cycle checkpoint kinases serve as important therapeutic targets for various cancers. When they are inhibited by small molecules, checkpoint abrogation can induce cell death or further sensitize ...

To address the roles of these interactions, we utilized single-molecule fluorescence assays to monitor Cse4 during kinetochore assembly. We found that Okp1/Ame1 and Scm3 independently stabilize Cse4 ...

Get Instant Summarized Text (Gist) A new real-time fluorescence detection technology has been developed for monitoring methylglyoxal, a compound affecting wine flavor and linked to diabetes risk ...

Western blot analysis was performed to examine autophagy and associated signaling proteins, while autophagy flux and autophagosome formation were visualized using fluorescence microscopy ... followed ...

AI-designed esmGFP protein could not have evolved naturally Scientists say esmGFP took 500M years’ worth of mutations to design AI-driven protein engineering could advance medicine and drug discovery ...

This is not the line used in the Sanger zebrafish sequencing project. Zebrafish strain in which macrophages are labelled with mCherry and neutrophils are labelled with GFP. Macrophages can ...