A further purification step using cation-exchange chromatography (CEX) gave 18 fractions of 15-280 mg each, on which reduction and alkylation steps were performed. Each fraction was then subjected ...

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The peptides were first separated into 80 fractions with a gradient of 2–60% acetonitrile in 10 mM ammonium bicarbonate pH 10 over 80 min. The peptides were combined into 18 fractions and dried using ...