Perforin forms transmembrane pores in the plasma membrane, allowing granzymes to enter the target cell cytosol and trigger ...

Despite recent therapeutic breakthroughs, cancer patients continue to face high recurrence and mortality rates due to treatment resistance. Cancer stem cells (CSCs), a subpopulation with self-renewal ...

By inhibiting the DNA polymerases, BKC enhances the endogenous RS in cancer cells, leading to increased DNA damage. IBC specifically inhibits CHK2 to prevent DNA repair. The combined use of BKC and ...

The MTT (thiazolyl blue tetrazolium bromide, catalog number A600799, Sangon) assay to measure cell proliferation ... To further characterize our KO cells, we obtained the top 11 potential off-target ...

Citation: How cells repair DNA's protective barrier: Addressing a rare genetic disorder characterized by rapid aging in children (2024, December 13) retrieved 8 January 2025 from https ...

metabolic flux analysis using 13 C-labeled glucose tracing assays and a Seahorse analyzer, as well as pharmacological approaches. In both human and mouse T cells that were activated under hypoxia ...

Moreover, we also demonstrate how the modularity of the cell-based SIMPL2 assay platform makes it a valuable tool for characterizing PPI ... biochemical techniques are accompanied by certain ...

HEX-labeled assays against ITR-2 (Bio-Rad, dEXD23004642), the CMV promoter (Bio-Rad, dEXD96423937), and eGFP (Bio-Rad, dCGTS863952655) were taken from the Bio-Rad Cell and Gene Therapy Assay ...

Potency assay will leverage the product's mechanism of action to define the biological activity. The absence of rigorous mechanistic data for the majority of cell and gene therapy therapeutics ...

We were pleased to accept 9 reviews, primary reports, and a meta-analysis on wide-ranging Research Topic from renal cell signaling mechanisms and approaches to study ... terminal deoxynucleotidyl ...