In principle, cloning PCR products necessitates some fancy ... much more robust behavioral assays need to be developed for screening.” Box 1: Gene trapping for the future Gene trapping is ...

Figure 1: Retroviral backbone of pSHAG-MAGIC2 ... in length from the coding sequence of the gene of interest for each clone to be constructed. Only coding sequences are targeted, and each shRNA ...

It allows for the cloning of multiple fragments directly into any vector without the use of restriction enzymes. Gibson Assembly Primer-Bridge End Joining (PBnJTM) allows a single primer or primer ...