the team knocked down each candidate gene individually and visualized changes in the mitochondria-actin interaction using a GFP-labeled probe. “It turned out all eight candidates showed an effect and ...

Mitochondria in the same cells were tagged with a GFP construct in which residues 1–52 of citrate synthase (a mitochondrial enzyme) were fused to the amino terminus of GFP (middle panel).

This is especially challenging for organelles like mitochondria. To address this, the researchers present, for the first time, a technique for targeted protein degradation within the mitochondria ...