Briefly, PCR was performed with 2 × KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Inc., United States) under the following conditions: initial denaturation at 95°C for 15 min, followed by 30 cycles ...
A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.
Because of the prominence of real-time PCR, companies are flooding the market with newer, improved reagents for every step of the process, from sample preparation to reverse transcription to ...
Include as controls 1 μl of the starting genomic DNA (Step 1) and 100 ng of primary selected ... region carried in the BAC (for example, a PCR fragment of a few hundred base pairs).
Many believe that achieving 10,000 steps daily is the standard for good health, but studies show that the average American only walks about 4,000 to 5,000 steps daily. The ideal number of steps varies ...