Huch’s pancreatic organoids are now a promising avenue for disease modeling and generating precision medicines to treat ...

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The cells were permeabilized with 0.05% Triton X-100 (Daejung Chemicals and Metals) at room temperature for 5 min. Next, the cells were stained with TRAP staining solution at 37°C for 20 min in ...

To this end, we selected 5 μM and 10 μM as low and high concentrations of STG for testing. Following the induction treatment, we conducted staining with rhodamine-labeled phalloidin. Similar to the ...

Yet achieving reliable, high-quality images can be challenging due to complex sample preparation and variable staining outcomes. This updated handbook contains a comprehensive collection of protocols ...

We observed that although the lamina co-localized with phalloidin, the NPC staining formed a separate layer of fragmented appearance up to 500 nm ‘above’ the F-actin shell (Figure 3A,B). Thus, the ...