Following washout with PBS (10 min, 3 times), cells were incubated with secondary antibodies for 1h and washed with PBS (10 min, 3 times). The cover slips were mounted onto frosted slides in a ...
Introduction: Traditional Miao and Dai Chinese medicines have used nicotine-rich leaf tobacco to treat traumatic injuries by promoting hemostasis. While nicotine is known to enhance platelet ...
Abstract: Maxwell's equations are replaced by a set of finite difference equations. It is shown that if one chooses the field points appropriately, the set of finite difference equations is applicable ...
Over the next four years, all indications are that Donald Trump and Benjamin Netanyahu may extinguish any possibility of a two-state solution in the Middle East. Many of Israel's far-right ...
Zona pellucida was removed by brief incubation in prewarmed acidic Tyrode’s solution. ICM was obtained by immunosurgery. Briefly, blastocysts were incubated in 20% rabbit anti-mouse whole serum (Sigma ...
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Neuroligins are postsynaptic cell-adhesion ...
San Diego, CA, United States) were maintained in growth medium (GM) that was prepared by supplementing Dulbecco’s Modified Eagle Medium (DMEM, D6171, Sigma) with 10% FBS (10270-106, Gibco, Grand ...