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San Diego, CA, United States) were maintained in growth medium (GM) that was prepared by supplementing Dulbecco’s Modified Eagle Medium (DMEM, D6171, Sigma) with 10% FBS (10270-106, Gibco, Grand ...
Paul G.A. Volders (Maastricht University, Netherlands; pIRES2_EGFP). Sildenafil and BaCl 2 were added to the Tyrode’s solution to obtain the final concentrations, 0.1, 1, and 10 μM in the case of ...
Whenever someone agrees wholeheartedly with something I write, I die a little inside. I know opinion columnists are supposed to be in the persuasion business, and that makes agreement the coin of ...
Xcellence RT imaging software (Olympus) controlled the microscope and image acquisition. Coverslips were perfused with Tyrode’s solution (119 mM NaCl, 2.5 mM KCl, 2 mM CaCl2-2H2O, 2mM MgCl2-6H2O, 25 ...