For the Cd3γ targeting vector, the humanizing chimeric CD3γ cDNA was introduced into the murine exon 3 by DNA synthesis. A loxP-flanked puromycin resistance cassette was inserted in 3’ of the ...

Colon scrapings were collected on ice and stored for a short term at −80°C. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). First-strand cDNA synthesis was performed using oligo(dT) ...

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These categories of experiments could help scientists, editors, and reviewers in deciding whether their proposed, ongoing work, or submitted manuscript is DURC. DNA synthesis technologies are ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. To address a wide range of genetic diseases, ...

在人类解码遗传信息的历史上，弗朗西斯·克里克（Francis Crick）在1957年首次提出中心法则，为遗传信息在生物大分子之间传递的顺序构建一个框架：DNA→RNA→蛋白质。

Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, California 94305, United States ...