Quantitative PCR was performed using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme; Q712-02) on the basis of the manufacturer’s instructions. For the quantification of mRNA levels, the ΔΔCT ...

(I) qPCR analysis of AQP1 gene expression in HAECs treated with 0.01% DMSO ... To identify genomic lesions, the following primers were used: aqp1a1-fwd (5’-CGCCTCCAGATTCATTAGCAGGA-3’) and aqp1a1-rev ...