Aβ 1-42 increased mRNA levels of SREBP1 target genes (acaca, acly, elovl6, and fasn) in microglia compared with control cells; this effect could be inhibited by 10 μM capsaicin or 1 μM shikonin ...

However, the mRNA expression of ACC2 and SREBP-1 (a master regulator of FA synthesis) remained unaltered in both MASLD-HCC (F0F1-F2 and F3F4), regardless the degree of fibrosis.

Relative PTGS2 mRNA levels were determined by real-time PCR. (D) Cells were treated with 30 μM 25-OHC in the presence or absence of 5 μM ferrostatin-1 for 24 h, followed by incubation with 10 μM Liperfluo for 1 h.

Sterol regulatory element-binding protein 1 (SREBP1) is part of the SREBP family and is a transcription factor that contributes to fatty acid synthesis. In addition to its role in lipid metabolism, SREBP1 has been demonstrated to play an oncogenic role in cancers [6, 7].

HCBP6 mRNA and protein expression levels significantly decreased in si- HCBP6 -transfected cells (Fig. 2A, C). Moreover, results of qRT-PCR and western blotting showed that SREBP1c, ACC1, p-ACC1, and FASN expression decreased significantly in si- HCBP6 -transfected cells, which was consistent with the decrease in HCBP6 expression (P < 0.05; Fig ...