Alcian blue (/ ˈ æ l ʃ ə n /) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the …

The alcian blue stains sialomucins, sulfomucins, and proteoglycans blue. Neutral mucins are stained deep red/magenta with the PAS. Tissues and cells that contain both neutral and acidic mucins will stain varying shades of purple due to the binding of …

The 3% acetic acid solution (pH2.5), Alcian blue stains both sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

2023年7月11日 · Staining Pattern: Alcian Blue 2.5- All Acid Mucins will stain bright blue. These include sulfomucins, sialimucins, proteoglycans; Alcian Blue 1.0- The most acidic mucins will stain bright blue. Biochemistry Theory: Alcian Blue 2.5 demonstrates acid mucopolysaccharides. A 3% acetic acid solution is added to the Alcian Blue solution to bring the ...

2021年5月4日 · Alcian Blue is a stain that is used to visualize acidic epithelial and connective tissue mucins. Mucins are a type of carbohydrate and are found in the GI tract and respiratory tract. Acidic mucins have a negative charge.

2022年7月7日 · The alcian blue stain causes acid mucins and mucosubstances to appear blue, and nuclei to appear reddish pink when counterstain neutral red is used. Alcian blue dyes are water soluble, and appear blue as they contain copper.

Alcian blue is a large conjugated dye molecule that initially was used for the dyeing of textile fibers. It is composed of a central copper-containing phthalocyanine ring linked to four isothiouronium groups via thioether bonds (Scott et al. 1964).

The combination of the Alcian blue and the PAS techniques can be used as a means of distinguishing neutral mucins from acid mucins. In most protocols, sections are stained with the standard Alcian blue (pH 2.5) method followed by the PAS technique. The Alcian blue at a pH of 2.5 will stain all acid mucins deep blue but will not color the ...