Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides (such as glycogen) and mucosubstances (such as glycoproteins, glycolipids and mucins) in tissues.

2021年9月14日 · PAS stain demonstrates polysaccharides, mucin, glycogen, certain glycoproteins and glycolipids, basement membrane and certain fungus in tissues. PAS helps to demonstrate glycogen, cellulose and starch. This is useful to detect glycogen deposits in liver when glycogen storage disease is suspected.

2022年6月21日 · Periodic acid-Schiff (PAS) is a staining technique for demonstrating the carbohydrates and fungal cell wall components. PAS can detect the presence of glycogen, polysaccharides, and mucin in the tissue that is either formalin-fixed, paraffin-embedded, or frozen tissue sections.

2022年6月22日 · Periodic-Acid Schiff (PAS) staining technique is used in histochemistry and histological studies to demonstrate the presence of carbohydrates and carbohydrate compounds such as polysaccharides, mucin, glycogen, and fungal cell wall components.

Periodic acid Schiff (PAS) stain is a method used to identify glycogen in tissues. PAS works by selectively oxidizing carbohydrate radicals, such as those found in glycols and related compounds, to produce dialdehydes. These dialdehydes react with Schiff’s reagent to produce a purple-magenta color.

2023年12月5日 · Principle of Periodic acid–Schiff (PAS) staining. The periodic acid reacts to oxidize the vicinal diols in these sugars (polysaccharides) and breaks up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units.

2024年5月19日 · Periodic Acid-Schiff (PAS) staining is a laboratory staining technique used to detect the presence of specific sugars and carbohydrates in tissue samples. The PAS stain highlights the presence of sugars, glycoproteins, and mucopolysaccharides in …

The PAS stain is used for demonstration of basement membranes, fungus secreting adenocarcinoma from undifferentiated squamous cell carcinoma, and mucosubstances secreted from the epithelia of various organs. A routine stain for liver and kidney biopsies.

Periodic acid–Schiff stains the fungal cell wall, and fungi appear pink. The PAS stain is very useful for detection of yeasts and hyphae. Many other staining techniques are available, including methylene blue, Giemsa, and calcofluor white stains.

2024年8月14日 · The Periodic Acid-Schiff (PAS) Stain is widely used technique in histopathology for the demonstration of carbohydrates and carbohydrate rich compounds in tissues.