A GPCR-based yeast biosensor for biomedical, biotechnological, …
2022年6月27日 · Here, we exploit the remarkable sensing capacity of G-protein coupled receptors to construct yeast-based biosensors for real-life applications. To establish proof-of-concept, we focus on...
TRUPATH, an open-source biosensor platform for interrogating the GPCR …
2020年5月4日 · To address this gap, we have developed an open-source suite of 14 optimized bioluminescence resonance energy transfer (BRET) Gαβγ biosensors (named TRUPATH) to interrogate the transducerome with...
ONE-GO: Direct detection of context-dependent GPCR activity
2024年4月30日 · In a recent study published in Cell, Janicot et al. introduced a universal biosensor platform capable of detecting GPCR signaling at endogenous levels, which facilitates its application across...
Direct interrogation of context-dependent GPCR activity with a ...
2024年3月14日 · Here, we developed a collection of compact ONE vector G-protein Optical (ONE-GO) biosensor constructs as a scalable platform that can be conveniently deployed to measure G-protein activation by virtually any GPCR with high fidelity even when expressed endogenously in primary cells.
Protocol for detecting endogenous GPCR activity in primary cell ...
2024年12月20日 · ONE vector G protein Optical (ONE-GO) biosensors can measure the activity of endogenously expressed G protein-coupled receptors (GPCRs) in primary cells. By detecting G proteins that belong to all four families (G s, G i/o, G q/11, G 12/13) across cell types, these biosensors provide high experimental versatility.
Genetically encoded fluorescent biosensors for GPCR research
2022年9月28日 · We will introduce fluorescent GPCR biosensors based on a variety of strategies such as fluorescent resonance energy transfer (FRET), bioluminescence resonance energy transfer (BRET), circular permuted fluorescent protein (cpFP), and nanobody. We will discuss the pros and cons of these GPCR biosensors as well as their applications in GPCR research.
Direct interrogation of context-dependent GPCR activity with a ...
2024年3月14日 · Here, we developed a collection of compact ONE vector G-protein Optical (ONE-GO) biosensor constructs as a scalable platform that can be conveniently deployed to measure G-protein activation by virtually any GPCR with high fidelity even when expressed endogenously in primary cells.
Advancements in GPCR biosensors to study GPCR-G Protein …
A hallmark of heterotrimeric G protein activation by GPCRs is the rearrangement or dissociation of the Gα and Gβγ subunits. The most consistent design format for Gα/Gβγ-based GPCR biosensors utilizes FRET or BRET components inserted or fused to some part of the Gα and to either the Gβ or Gγ 2 (Figure 1F, Table 1, Figure 2). High ...
Advancements in G protein-coupled receptor biosensors to study GPCR …
Enzymatic and cellular signalling biosensors are used to decipher the activities of complex biological systems. Biosensors for monitoring G protein-coupled receptors (GPCRs), the most drugged class of proteins in the human body, are plentiful and vary in utility, form and function.
Genetically-encoded biosensors for analyzing and controlling …
2020年8月1日 · GPCR-based sensor provides a versatile and modular toolset to rewire cell signaling. Optogenetic sensor directs protein assembly and removes rate-limiting steps. Yeast has been a robust platform to manufacture a broad range of biofuels, commodity chemicals, natural products and pharmaceuticals.