2017年5月26日 · Interpreting the results of indirect immunofluorescence. The immunofluorescent slides are examined to determine the presence of autoantibodies via the patterns of immune deposition. The results are subjective and indirect immunofluorescence cannot be used reliably to monitor disease severity and its treatment.

A cross-sectional study of direct immunofluorescence in the diagnosis of immunobullous dermatoses. Indian J Dermatol 2014; 59: 364–8. DOI: 10.4103/0019-5154.135488. PubMed Central; On DermNet. Biopsy of skin; Dermatopathology; Dermatopathology (CME course) Allergies explained; Blistering skin conditions; Indirect immunofluorescence; Other ...

Direct immunofluorescence shows linear deposition of IgG (most often IgG4 subtype) and C3 along the basement membrane (figure 4). Split skin indirect immunofluorescence studies are generally not needed, but may be useful in rare cases when epidermolysis bullosa aquisita is suspected. Differential diagnosis of bullous pemphigoid

Serum autoantibodies detected by the indirect immunofluorescence test or enzyme-linked immunosorbent assay (ELISA) are against type VII collagen in type I bullous SLE. BP 230, BP 180, or laminin 5 or 6 are detected in type II bullous SLE.

However direct immunofluorescence (DIF) shows a speckled pattern of IgG in the lower one third of the epithelium (basal and parabasal cell layers). Indirect immunofluorescence (IIF) on a blood sample detects an IgG and/or IgA autoantibody against keratinocyte nuclei in skin and guinea pig oesophageal mucosa but not in HEp2 cells or kidney.

Diagnosis of chronic blistering diseases often requires skin biopsy for histopathology and direct immunofluorescence. A blood test for specific antibodies (indirect immunofluorescence) may also prove helpful in making the diagnosis of an immunobullous disease. Epidermolysis bullosa. Various types including acquired and genetic forms

Direct immunofluorescence staining of a skin biopsy taken adjacent to a blister highlights antibodies along the basement membrane that lies between the epidermis and dermis. Blood tests include an indirect immunofluorescence test for circulating pemphigoid BP180 antibodies. Immunoblotting. Other tests will relate to planning and monitoring ...

Blood tests — indirect immunofluorescence for circulating autoantibodies can be used to distinguish pemphigoid gestationis from other autoimmune blistering disorders; What is the differential diagnosis for pemphigoid gestationis? Polymorphic eruption of pregnancy; Pustular psoriasis of pregnancy; Bullous pemphigoid; Epidermolysis bullosa ...

In 20 to 30% of the patients with Brunsting-Perry cicatricial pemphigoid, skin antibodies can be detected in their bloodstream. (positive indirect immunofluorescence). Clinical presentation of Brunsting-Perry cicatricial pemphigoid. The diagnosis of Brunsting-Perry cicatricial pemphigoid is based on the following features:

Immunofluorescence is negative. Pemphigus foliaceus: Acantholysis more superficial within the granular layer. Lacks mucous membrane involvement. Grover disease: There is a pemphigus-like form of this disease. Negative immunofluorescence and lack of …