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  1. ScienceDirect
    https://www.sciencedirect.com › science › article › pii

    Article A systematic survey of PRMT interactomes reveals the key …

    • Thousands of proteins undergo arginine methylation, a widespread post-translational modification catalyzed by several protein arginine methyltransferases (PRMTs). However, global underst… 展开

    1. Introduction

    Arginine N-methylation is catalyzed by a class of enzymes known as protein arginine … 展开

    ScienceDirect
    2. Materials and methods

    2.1. Plasmids
    For identifying the interacting proteins of each PRMT, human PRMT1–PRMT9 were amplified by polymerase chain reaction (PCR) and insert… 展开

    ScienceDirect
    3. Results

    3.1. Identification of the interacting proteins of each PRMT
    As protein modification enzymes, PRMTs dynamically interact with their protein substrates, com… 展开

    ScienceDirect
    4. Discussion and conclusion

    Although a common PTM, arginine methylation is a relatively understudied modification. Numerous proteins undergo methylation at their arginine residues [5], [7], [65]. Several … 展开

    ScienceDirect
    Acknowledgments

    We thank Han Yan at Omics Core of Bio-Med Big Data Center, CAS-MPG Partner Institute for Computational Biology (PICB), for assistance with next-generation sequencing, Yu-… 展开

    ScienceDirect
    What is the difference between PRMT1 and PRMT5 methylation?
    In vitro methylation assays on a subset of identified PRMT5 targets led to the refinement of the consensus motif targeted by PRMT5, with the substrate arginine often surrounded by glycines. This is different from PRMT1 that is more permissive regarding the surrounding amino acids in the targeted motif.
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    目录
    1. Introduction
    2. Materials and methods
    3. Results
    4. Discussion and conclusion
    Acknowledgments
    What is the difference between PRMT1 and PRMT5 methylation?
     
  1. Science | AAAS
    https://www.science.org › doi

    Proteomics profiling of arginine methylation defines …

    Protein arginine methyltransferases (PRMTs) catalyze arginine methylation on both chromatin-bound and cytoplasmic proteins. Accumulating evidence supports the involvement of PRMT5, the major type II PRMT, in cell survival and …

  2. CSDN博客
    https://blog.csdn.net › coffeeii › article › details

    生信学习之通路富集一（GO分析） - CSDN博客

    2023年5月6日 · go富集分析是一种常用的生物信息学方法，用于确定一组基因在特定的生物学过程、细胞组分或分子功能中的富集情况。 在R语言中，可以使用多个包来进行GO 富集 分析 。

  3. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov › pmc › articles

    Protein Arginine Methyltransferase 5 Functions

    2021年8月27日 · Functional GO and KEGG pathway enrichment analysis of PRMT5 interacting proteins. (A) Plots of significantly enriched GO terms of PRMT5 interacting proteins for …

  4. Nature
    https://www.nature.com › articles

    PRMT5-regulated splicing of DNA repair genes drives ... - Nature

    2024年12月18日 · GO analysis highlighted “cell adhesion” as the most significantly enriched GO term for GSK591-dependent DEGs (Fig. 6C).

  5. Nature
    https://www.nature.com › articles

    PRMT1-dependent regulation of RNA metabolism and DNA …

    2021年7月30日 · To gain insight into the potential cellular functions of PRMT1 substrates, we performed Gene Ontology (GO) analysis after mapping peptides back onto their protein of origin.

  6. 其他用户还问了以下问题
    Is there a competition between PRMT1 and PRMT5?
    Hence, in light of the possible competition between PRMT1 and PRMT5 for the same sites on nonhistone proteins, several RGG sequences are likely present as both ADMA and SDMA: these two modifications are isobaric and thus undistinguishable in the survey scan (MS1).

    Proteomics profiling of arginine methylation defines PRMT5 substrate

    science.org
    What does PRMT5 do?
    As a protein arginine methyltransferase, PRMT5 catalyzes the methylation of targeted proteins, and either inhibits the transcription of tumor suppressors (such as p53, E2F-1, ST7, NM23, and CASP4) to promote tumor occurrence, ( Pal et al., 2004; Zhu and Rui, 2019) or is involved in the activation of transcription factors.

    Protein Arginine Methyltransferase 5 Functions via Interacting Proteins

    ncbi.nlm.nih.gov
    What is the difference between PRMT1 and PRMT5 methylation?
    In vitro methylation assays on a subset of identified PRMT5 targets led to the refinement of the consensus motif targeted by PRMT5, with the substrate arginine often surrounded by glycines. This is different from PRMT1 that is more permissive regarding the surrounding amino acids in the targeted motif.

    Proteomics profiling of arginine methylation defines PRMT5 substrate

    science.org
    What is the role of PRMT5 in RNA posttranscriptional processing?
    It also led to the identification of novel PRMT5 targets, all involved in RNA posttranscriptional processing. In vitro methylation assays on a subset of identified PRMT5 targets led to the refinement of the consensus motif targeted by PRMT5, with the substrate arginine often surrounded by glycines.

    Proteomics profiling of arginine methylation defines PRMT5 substrate

    science.org
    Does oligomerization increase PRMT1 activity?
    By chemical crosslinking, we previously found that PRMT1 exists in different oligomer forms and oligomerization is concentration-dependent and biochemical analysis showed that PRMT1 activity increases as a result of the oligomerization, though the mechanism is unknown (20).

    Oligomerization of protein arginine methyltransferase 1 and its

    sciencedirect.com
    How do we identify putative interactors of a PRMT?
    We identified putative interactors of each PRMT by evaluating protein quantification values, and scored the positive hits using an LFQ intensity cutoff of two-fold higher than that of the mock control (see Supplementary materials and Table S1 online).

    A systematic survey of PRMT interactomes reveals the key roles of

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  7. ScienceDirect
    https://www.sciencedirect.com › science › article › pii

    Oligomerization of protein arginine methyltransferase 1 and its ...

    2024年12月1日 · This work is to investigate mechanisms of PRMT oligomerization using PRMT1 as a model enzyme, which is the predominant type-I PRMT member that is responsible for the …

  8. ResearchGate
    https://www.researchgate.net › figure › Functional-GO...

    | Functional GO and KEGG pathway enrichment analysis of PRMT5 ...

    The analysis presented in this study provides an initial description of the proteins specifically immunoprecipitated by a PRMT5-specific antibody but not by a non-specific IgG.

  9. ResearchGate
    https://www.researchgate.net › figure › GO-and-KEGG...

    GO and KEGG analysis of DEGs in LC cell overexpressing PRMT5.

    Download scientific diagram | GO and KEGG analysis of DEGs in LC cell overexpressing PRMT5. (A, B) The heatmap and volcano plot of 3137 DEGs between OE and WT groups. (C) PRMT5 …

  10. Nature
    https://www.nature.com › articles

    Profiling PRMT methylome reveals roles of hnRNPA1 arginine

    2021年3月29日 · f–h Gene ontology (GO) (f), KEGG pathway (g), and CORUM 47 (h) analysis using Metascape 46 for PRMT7 methylome. Representative terms from the top 20 enriched …